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Wherever possible, calibrate with defined oxidant fluxes (enzymatic generators), internal standards, or ratiometric reporters and fully report matrix and environmental conditions (buffer composition, protein content, temperature, pH, oxygen tension, light exposure) plus acquisition timing/kinetics to separate bursts from steady states (Kalyanaraman et al

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Common iron quantification methods such as Quantitative susceptibility mapping [23,28,46] , an MRI modality sensitive to Fe 3+ , or ICP-MS, that measures total iron [41,47] , are not capable of differentiating between pro-ferroptotic labile Fe 2+ and functionally essential intracellular iron, such as that required for mitochondrial ATP production