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Description

Samples were loaded into 5 L standard-treated capillaries (Hirschmann, Germany) after the reactions were incubated in the dark at 25 C for 30 min to 1 h

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Unsupervised hierarchical cluster analysis and supervised orthogonal partial least squares discriminant analysis (OPLS-DA) were performed using the Metaboanalyst 5.0 web server 45 (accessed on August 2022) Variable Importance in Projection (VIP) scores greater than 1 was considered to select relevant variables in the models 46

Injectable peptides carry their own risks: infection at the injection site, dose errors during reconstitution, and contamination from non-pharmaceutical-grade sources