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Tissues were homogenized and lysed in T-per Tissue protein extraction reagent (78510, Thermo Fisher Scientific) using a bead homogenizer for 3 min at maximum speed (Qiagen, TissueLyser II) for total tissue protein

Campbell JD, et al

We hypothesized that patients with stable schizophrenia would exhibit a reduction in glutathione, glutamate, and/or glutamine in the cerebral cortex, consistent with a post-inflammatory response, and that this reduction would be most marked in patients with residual schizophrenia, in whom an early stage with positive psychotic symptoms has progressed to a late stage characterized by long-term negative symptoms and impairments

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Organs can be classified as producers or consumers of glutamine skeletal muscles, lungs, liver, brain and adipose tissue present high activity of glutamine synthetase (an enzyme that synthesizes glutamine from ammonia and glutamate in the presence of adenosine triphosphate-ATP) and are considered as glutamine producers