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While glutathione was once thought to be the major driver of ER oxidative folding, more recently a functional role for GSSG in the ER lumen has fallen out of favor, as impairment of glutathione synthesis in yeast (Frand and Kaiser, 1998) and ER glutathione depletion in mammalian cells (Tsunoda et al., 2014) failed to compromise ER protein oxidation

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