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In detail, bEnd3 cells were seeded in a 6-well plate and incubated overnight to reach 60-70% confluency

GSH was indeed shown to permeate the ER membrane

The levels of cysteine and GSH were slightly decreased in cells expressing 3MST in the presence of Na 2 S, but not in a statistically significant quantity (Supplementary Fig

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We reiterate that suppliers who dispense a drug, but do not actually administer the drug, are not expected to monitor or bill for discarded amounts that are discarded after the drug is supplied because they are not at the site of administration to measure discarded amounts