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For differentiation, the hPSC aggregates were collected from the flask, transferred to a 50 ml conical tube and pelleted by centrifugation for 5 minutes at 300 x g at room temperature

By knocking out the fatty alcohol oxidation gene, HFD1 , and inducing the overexpression of alcohol dehydrogenase and aldehyde reductase, the fatty alcohol yield can reach 1.5 g/L (Zhou et al., 2016)

This makes it unlikely that any human material remains in the final vaccine

Only 2 livers developed methaemoglobinaemia (peak MetHb of 6%), with an average onset of 116.5 h

The primary challenge is that the body begins to decompose, a process that can naturally produce alcohol

Shaking creates foam at the air-liquid interface, which can denature peptide molecules