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Recombinant strains exhibited a differential response during SSF with 10% solids As there were no discernible differences in the physiology of the recombinant strains during SSF using 5% solids, the WIS content was increased to 10% under the hypothesis that a higher concentration of inhibitors present in the raw material would enable us to delineate the phenotypic differences between the strains without killing the cells rapidly

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47 Recommendation 3.1 Genetic counseling should be provided to parents with affected children and the 25% recurrence risk with regard to future pregnancies should be discussed in detail