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In addition, processed samples need to be frozen immediately and must always be protected from direct light [18,19]

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The tumor sizes were monitored by caliper for three consecutive weeks

doi: 10.1093/annonc/mdz394.048 235 CiomborKKWhisenantJCardinDBGoffLWDasSSchulteMet al

The pH was adjusted to 6.5.Procedure: The medium for the estimation was prepared as shown below and was allowed to run for 60 seconds each time before the absorbance was read against the blank at 340nm