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The washing buffer may contain low concentrations of non-ionic detergents or mild salts to enhance the removal of loosely bound contaminants without disrupting the GST-glutathione interaction

Recognized as a biotechnology-friendly microbe, Escherichia coli has been commonly applied for the mass production of recombinant proteins

Adverse events were considered related to the treatment if they started or worsened following the start of the trial

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Mild pain and swelling at the injection site can be eased by applying an ice pack for 1020 minutes as needed/when necessary

10.1111/j.1471-4159.2009.06319.x [DOI] [PMC free article] [PubMed] [Google Scholar] Chai Y.-C., Mieyal J