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miRNA interplay: mechanisms and consequences in cancer

Autism 6(3):315329

Crome P, et al

We used apyrase to hydrolyze GDP in order to form stable nucleotide-free complexes

To this end, we deleted SLC25A45 in HEK293 cells using a CRISPR system in which the cDNA sequence of human SLC25A45 was reintroduced (KO + SLC25A45, herein rescue cells) ( Fig