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Cell culture and cytotoxicity detection The human lens epithelial cell line (HLEB3) was cultured in Dulbeccos Modified Eagles Medium (DMEM) with 10% fetal bovine serum (FBS) and maintained in a 37 C incubator with 5% CO 2

Edited by Professor Nickolai Barlev Supplementary information Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made

Numerous studies have reported that vitamin C has anticancer properties and can enhance the efficacy of antitumor treatments (33,153)

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10.1038/s41467-024-50465-z 8 BRAIN Initiative Cell Census Network (BICCN) (2021)

Charting the development timeline of these age-hallmarking pathological processes might pave the way toward more tailored and effective therapeutics that specifically target the most essential process, at the most appropriate time and in the most suitable patients