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This step was followed by fixation in 4% paraformaldehyde for a duration of 24 h

Next, we tested the interaction in Drosophila S2R+ cells by co-immunoprecipitation under native conditions (Figure 2A)

Wallingford, UK: CAB International

Our results suggest that colchicine promotes the binding of HBc to the autophagy receptor p62, leading to the recruitment of p62 and LC3 to initiate autophagy