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Induction of kidney IRI was performed via a midline abdominal incision and a bilateral renal pedicle clamping for either 40 min (severe IRI, or lethal-to-WT IRI) or 50 min (ultra-severe IRI) using microaneurysm clamps (Aesculab)

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Subsequently, colonies were fixed with 4% paraformaldehyde for 25 min and stained with 0.2% crystal violet solution for 30 min at room temperature (25-30C)

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10.1016/j.neulet.2020.135268 76 IzumiY.SawadaH.YamamotoN.KumeT.KatsukiH.ShimohamaS.et al

Potential use of Starmerella bacillaris as fermentation starter for the production of low-alcohol beverages obtained from unripe grapes