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5 Discussion 5.1 Discussion of experimental methodology In validation experiments, joint cavity effusion and tophi showed complex and distinct protein compositions

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The western blot was performed using a GPX4 antibody (Abcam, [EPNCIR144], ab125066) (1:1000), -actin antibody (Abcam, ab8227) (1:5000) for loading control, and goat anti-rabbit IgG (H + L) secondary antibody, HRP (ThermoFisher Scientific, 31460) (1:3000)

Ethics statement The studies involving human participants were reviewed and approved by CAMH Research Ethics Board

Scientists hoped to use these newfound peptides to combat melanoma by stimulating the bodys natural pigmentary mechanism to create a tan without first needing exposure to harmful levels of UV radiation