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Postnatal cortical neuron isolation and culture Postnatal cortical neurons were isolated following a protocol adapted from Brewer and Torricelli (2007) and the Miltenyi Biotec MACS protocol for the isolation and cultivation of neurons from adult mouse brain

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1 h shaking, 15 min quenching) successfully conjugates to anti- TP53 antibody, forming a chemiluminescent immunoconjugate with a minimum detectable sensitivity of 1.8 10 -17 mol [1]

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