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The beads were then pelleted and washed in H buffer, and bound proteins were subjected to immunoblot analysis

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Colon tissues protein samples were prepared with RIPA lysis buffer supplemented with protease inhibitor and phosphatase inhibitor (Beyotime, China) for 30 min on ice (RIPA lysis buffer: protease inhibitor: phosphatase inhibitor = 50:1:1)

Glutathione compartmentalization and its role in glutathionylation and other regulatory processes of cellular pathways