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Non-drinker and non-smoker with admitted voice abuse patterns particularly while coaching sport

Approximately 50 mg of tissue was collected for determination of GSH content, as previously described

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Hereafter, 0.5 ml of non-clotted blood was transferred to a 2 ml vial and immediately centrifuged (3,000 g, 10 min), and the supernatant was subsequently removed

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PARP1 inhibitor (PJ34) improves the function of aging-induced endothelial progenitor cells by preserving intracellular NAD+ levels and increasing SIRT1 activity