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Bioinformatics identification of Ferroptosis-related biomarkers and therapeutic compounds in ischemic stroke

Journal of Biological Chemistry, 2007

Ghanei E, Baghani M, Moravvej H, Talebi A, Abdollahimajd F

Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation

Diagnosis is clinical and relies on recognizing a distinct hormonal pattern , supported by targeted testing and careful exclusion of other conditions

These are typically transient and resolve within minutes