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On longer flights with flexible itineraries, passengers can sometimes arrange ground stops specifically for smoking breaks

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Slides were then cooled to room temperature on ice and washed three times with TBST (1 TBS, 0.5% Triton X-100) before blocking in Block-B buffer (1 TBS, 3% BSA, and 0.1% Triton X-100) for 30 min at room temperature