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Overnight cultures were subcultured 1:100 into fresh LB medium supplemented with 50 g/mL kanamycin and grown at 37 C, with 230 rpm shaking until an OD 600 of ~0.8 was reached

Ethics statement The studies involving humans were approved by Ethics Committee of the Area Vasta Nord Ovest Toscana (Italy)

The pregnancy induced hypervolemia generally is considered beneficial for the pregnant woman as it maintains the hemodynamic stability with the blood loss at the time of delivery (Reference Carbillon, Uzan and Uzan25)

Figure 9A illustrates the operational principle of this aptasensor, where the binding between insulin and aptamers is detected via EIS

128 Disruption of gut barrier integrity and microbial translocation: The accelerator of liver disease progression Pathophysiological changes in gut barrier structure and function As a key physical and immunological defense line separating intestinal lumen contents from the hosts internal environment, the integrity of the gut barrier suffers systemic destruction during the progression of liver diseases

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