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Gradient started 90% B, decreased to 65% B at 10 min, further decreased to 20% B at 11.5 min, kept at 20% B until 13 min, returned to 90% B at 14 min and kept at 90% B until 20 min

Under normal physiological conditions, small amounts of 5-oxoproline are derived from gamma-glutamyl-cysteine by the action of gamma-glutamyl cyclotransferase (63)

For example, administration of the cysteine-donor N-acetyl cysteine (NAC) in humans significantly increases blood [6264] and muscle [65, 66] GSH levels, and in vitro studies show methionine supplementation to have beneficial effects on GSH hepatocyte concentrations [67]

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It is crucial to be aware of these potential effects, especially if you are considering adding supplements to your regimen