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-Tocopherol (30 mg/kg, i.p.) has shown beneficial effects in experimental renal ischemia, including reduction in tubular damage scores and cast formation, decreased blood urea nitrogen (BUN) and creatinine elevations, protection against oxidative DNA damage in renal tubular cells, and preservation of glomerular filtration capacity [53]

Treatments might include injections or high-dose oral supplements to restore B12 levels

Comet Assay for DNA damage The Comet Assay Kit (Trevigen, Gaithersburg, MD) was used to detect single- and double-stranded DNA breaks in cultured cells

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