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Immunofluorescence staining All cells on the sections were fixed with 4% paraformaldehyde, followed by washing three times in PBS and blocking with 5% BSA

10.1136/gutjnl-2015-309252 5

If you are somebody, who is struggling with uneven skin tone or other dermal problems such as hyperpigmentation or other dark spots are interfering with your appearance

When followed up from metformin initiation, 10% with the AA genotype were vitamin B 12 deficient by 11 years vs 21 years for 10% of the GG group

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