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In summary, it was a parallel randomized, double-blinded, and clinical controlled trial and was conducted et al.-Zahra Hospital, Isfahan, Iran

PCR was performed with a 2 l cDNA mixture in a total volume of 35 l using Taq polymerase and 0.2 nmol/l of each forward and reverse primer (intron spanning, 5CCCCCGATAGCTCCTACAAC3 and 3AAGGCCTTCCTCATGCACT5, TRPV1 mRNA accession number NM_018727) after initial 95 C for 3 min over 35 cycles of 95 C for 30 s, 64 C for 30 s and 72 C for 45 s, finalized by 72 C for 7 min and a temperature hold at 4 C in a Techne TC-512 gradient thermal cycler (Staffordshire, UK), yielding a 295 bp product

BAs function as signaling molecules through various receptors, including farnesoid X receptor (FXR), vitamin D receptor, pregnane X receptor and members of the G-protein-coupled receptor superfamily, to regulate their own synthesis as well as other metabolic processes, such as glucose, lipid and energy metabolism (Yu et al., 2018)

doi:10.3390/ijms241914841

2005) Magnesium and Vitamin A were also beneficial

Finally, the potential drug resistance may hinder the application of glutamine metabolism inhibitors for cancer treatment