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Molecular chaperones inhibit improper intermolecular interactions of protein intermediates, literally consuming ATP to untangle trapped intermediates

For analysis of GSSG, a 100 L aliquot of erythrocyte lysate or plasma protein-bound glutathione solution was treated with 2 L of 2-vinylpyridine as a 10% solution in KPE ( v/v ) at 22C for 1 h in a well-ventilated fume hood