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Beyond epithelial damage: vascular and endothelial contributions to idiopathic pulmonary fibrosis

It helps focus light onto the retina, allowing us to see clearly

Membranes were then incubated overnight at 4C with the following primary antibodies: NINJ1 (1:1000, Invitrogen, PA5-95755), GAPDH (1:1000, Cell Signaling, #97166), PANK1 (1:1000, Cell Signaling, #23887), xCT (1:1000, Cell Signaling, #12691), V5 tag (1:1000, Cell Signaling, #13202), and -tubulin (1:1000, Cell Signaling, #2128)

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We found that 2-HG levels accumulated across the continual stimulation time course, but were only slightly increased upon acute stimulation, peaking at 12 h (Figs