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Oral administration of allicin to mice lowered ROS production and prevented ROS-induced cardiac hypertrophy by inhibiting pro-inflammatory pathways like mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase 3 (GSK3) signaling pathways (20)

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Cells treated with the indicated siRNAs were analyzed by FACS for their cell cycle distribution after 30 min incubation with 10 M EdU to label cells in S phase (gating strategy illustrated in Supplementary Figure 1)

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