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To aliquot safely: Work in a clean environment, ideally with minimal air movement Use sterile technique throughout Prepare labeled sterile vials or containers in advance Draw from the master vial and dispense into individual containers Refrigerate all aliquots immediately The trade-off is that aliquoting introduces more handling steps where contamination can occur

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In humans, a study on a cohort of 49 healthy elderly subjects reported that the subjects maintained on a regimen of dietary restriction, in comparison to subjects maintained on ab libitum diet, exhibited enhanced verbal memory, albeit without increased serum levels of BDNF [182]

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