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More evidence exists that a single subunit, specifically homomeric receptors, can form functional channels, such as 7, 8, and 9

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For example, 6 subunit is exclusively expressed in cerebellar granule cells and the subunit is largely restricted to the retina

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After three washes with PBT, the sections were incubated with the Alexa Fluor 546-conjugated anti-rabbit (1:600) and Alexa Fluor 488-conjugated anti-mouse (1:400) secondary antibodies for 2 h at room temperature in the dark