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Using sterile water is better, but without the bacteriostatic agent, you risk contaminating your vial with every puncture

It is reported that the intervention of probiotics improved the gut microbiota and has an effective protective effect on the immune health of the host [26, 27]

Also, it reduces levels of malondialdehyde (MDA) and increases activity of superoxide dismutase (SOD) and catalase (CAT), which in turn reduces inflammation similar to psoriasis caused by imiquimod in mouse models (Zhang et al

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This reduction leads to an increase in reactive oxygen species (ROS) like hydroxyl radicals, hydrogen peroxide, and superoxide ions

ASC can act as a cofactor to keep iron (Fe) in the Fe 2+ state and protect 2-ODDs from oxidative damage (Myllyla et al., 1984