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Cells were transfected with double-stranded siRNAs (0.5 g ml 1 ) in antibiotic- and serum-free medium for 6 h by using the X-tremeGENE siRNA transfection reagent according to the protocol provided by manufacturer (Roche Applied Sciences, Indianapolis, IN, USA) and recovered by the addition of fresh medium containing 10% FBS for 12 h

doi: 10.1001/jama.282.1.31 106 MossR

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Although N1m-mRNA is less detectable by the toll-like receptors (TLR) 7 and 8 and considered a safer modality, it requires continued surveillance to address uncertainties around maternal-fetal impacts

Necroptosis in obesity: a complex cell death event

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