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Increased levels of DNA methylation of the Trim13 gene promoter in DKD result in lower production of Trim13, decreased proteasomal degradation and ubiquitination of CHOP, and increased collagen synthesis[45]

Beitler JR, Thompson BT, Baron RM, Bastarache JA, Denlinger LC, Esserman L, et al

Glutathione is involved in cellular metabolism and oxidises quickly

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The purified OT-1 CD8 + T cells were then maintained in DMEM-based culture medium supplemented with IL-2 (10 ng ml 1 , 212-12, PeproTech), T-Select H-2K OVA Tetramer (Medical & Biological Laboratories) and -mercaptoethanol (100 mM, M3148, Sigma-Aldrich) and cultured for 35 days