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Cells were fixed and permeabilized (4% formaldehyde and 0.1% Triton-X 100/PBS 5 min at room temperature), followed by blocking (PBS, 0.1% Tween 20, 1% BSA, and 10% normal goat serum for 1 h or overnight at room temperature)

Validation of GCMS method has been previously reported 20

The ANT-treated animals had significantly increased plasma levels of ALT, AST, and total bilirubin compared to the control group (Fig

In addition, a combination of PNM with porous degradable conduits may enhance regenerative capacity as compared to the silicone conduits used in the present study

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