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Cultures were passaged by trypsinization (0.05% trypsin-0.53 mM EDTA) every week and were seeded at a density of 1.2 10 4 cells/cm 2 in 60 20 mm or 100 20 mm diameter culture dishes (Sarsted, Nmbrecht, Germany), 96-well or 24-well plates (Sarstedt), or 15 -slide 4-well coverslip (Ibidi USA, ThermoFisher Scientific) for subsequent experiments

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Independently lab-tested for identity, purity and contaminants

While direct measurement of methemoglobin concentration will clinch the diagnosis, oxygen saturation differential between pulse oximetry reading and arterial blood gas measurement should alert the transplant physician

Although some articles reported an estimation of APAP or PAP toxicity in kidney cells, no study characterized the biological effects of cysteine conjugates of APAP or PAP in human kidney cells