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The refolding of SRCRD was performed through a dilution process with addition of the solubilized SRCRD sample to eightfold volume of refolding buffer (50 mM TrisHCl (pH 8.0), 200 mM NaCl, 1 M urea, 0.5 M l-Arg, 2 mM GSH, 0.4 mM GSSG, and 2.5 mM CaCl 2 ) in several batches

ROS released from mitochondria can activate the NLRP3 inflammasome, thereby facilitating the onset of pyroptosis

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This mechanism does not broadly stimulate cell death but instead targets a survival dependency characteristic of senescent cells

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