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During intestinal microbial dysbiosis, the imbalance in the composition of the bile acid pool disrupts the homeostatic regulation of the gut-kidney-bone axis, serving as an important mediating mechanism for the comorbidity of diseases such as CKD, osteoporosis (OP) and osteoarthritis

Nitrocellulose membranes were incubated with HRP-labeled secondary antibodies (1:2,000, Santa Cruz Biotechnology, Heidelberg, Germany) according to the species of primary anti-bodies and bands were detected using Clarity Western ECL substrate (Bio-Rad, Milan, Italy)