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Development of novel biosensors to study receptor-mediated activation of the G-protein alpha subunits G(s) and G(olf)

In addition to genetic approaches, a pharmacological tool that has frequently been used to deplete glutathione is buthion- ine sulphoximine (BSO), a specic inhibitor of g-ECS (Grif- th & Meister 1979)

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lowest tolerated dose Phase Step 2 Weeks Weeks 5-8 Cagrilintide Dose 0.5 mg/week Purpose Dose escalation Phase Step 3 Weeks Weeks 9-12 Cagrilintide Dose 1.0 mg/week Purpose GI side effects often peak here Phase Step 4 Weeks Weeks 13-16 Cagrilintide Dose 1.7 mg/week Purpose Approaching maintenance Phase Maintenance Weeks Weeks 17+ Cagrilintide Dose 2.4 mg/week Purpose Target dose used in late-stage study protocols Each step lasts 4 weeks

Both peptides have likewise been studied mostly in animal models, with limited human studies published to date