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The slides were mounted with a ProLong Diamond antifade reagent (Invitrogen, Thermo Fisher) and imaged with a laser fluorescence confocal microscope (LSM700, Zeiss)

Reapplication: Reapply every two hours, especially if exposed to direct sunlight, swimming, or sweating

165 In addition to lysine methyltransferases, elevated histone arginine methyltransferase PRMT5 level is also related to the malignant progression of tumor, and PRMT5 inhibition by GSK3186000A sensitized human AML cell lines to PARP inhibition

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