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A more accurate assessment of these values was not possible due to low solubility of these compounds at high micromolar concentrations

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The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol

They are found within the macula and surrounding tissues, including blood vessels and capillaries which nourish the retina (Rapp 2000)

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