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Intracellular ROS detection Raw264.7 cells (30-40% confluence) seeded onto a confocal dish were stimulated with lipopolysaccharide (LPS

The reaction was held in an autoclave for 48 h at 200C, followed by 15 min of sonication at room temperature

In contrast to mouse collagen organoids, which predominantly consisted of a single undifferentiated cell population, human collagen organoids exhibited two distinct clusters, annotated as cluster 1 and 2 (Figs

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