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In the context of limited glutamine, various cell components in the TME compete for glutamine, and tumor cells can increase their uptake of glutamine due to their unique metabolic mode, thus providing certain competitive advantages

Nature 451 , 11111115 (2008)

The supernatant was aspirated and the cell pellet was suspended in supplemented DMEM media for further experiments

This IV treatment addresses nutrient depletion caused by vomiting

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