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Lentivirus production for CRISPR-Cas9 screen 15 10 6 HEK-293T cells were seeded in T175 cm 2 flasks in DMEM (Thermo Fisher Scientific #12430054) supplemented with 10% fetal bovine serum (GeminiBio #100-106)

The reaction mixture (DHA) contained 90 mM potassium buffer with phosphate (pH 7.0), 12.5 mM glutathione reductase (GSH), 0.178 mM EDTA, 0.1 mM enzymes extract, and 1 mM dehydroascorbate

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