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A reasonable titration schedule might look like this: Week 1: 200mcg once daily Week 2: 300mcg once daily Week 3 onward: 300-500mcg based on response and tolerance Optimizing injection technique Poor injection technique contributes to injection site reactions and potentially to other effects like nausea from anxiety around the process

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J Neuroendocrinol

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Again consistent with the reduction in adipogenic transcription factors, all genes associated with fatty acid and triglyceride metabolism, such as adipocyte fatty acid binding protein, perilipin, lipoprotein lipase, glycerol-3-phosphate acyltransferase (GPAT), mitochondrial GPAT, acetyl-CoA carboxylase, fatty acid synthase, acyl-CoA synthetase, acyl-CoA:diacylglycerol acyltransferase 1 (DGAT-1), DGAT-2, ATP citrate lyase, were reduced in WAT of HSL null mice on either a normal chow or high fat diet [13, 17]