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salina was more effective than C

Differential centrifugation and isopycnic equilibration in WI-38 fibroblast density gradients allowed for GSH localization in all subcellular fractions, whereas glutathione peroxidase and reductase activities were restricted to cytoplasm and mitochondrial fractions

doi:10.1093/jn/130.4.988S

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The reaction mixture was bubbled with N 2 for 5 minutes at rt then heated to 100 C

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