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CRISPRCas9 gene editing of human and mouse naive CD4 + T cells Single-guide RNAs (sgRNAs) targeting human BDH1 (mC*mC*mG* rUrArC rUrGrC rArUrC rArCrC rArArG rUrUrG rUrUrU rUrArG rArGrC rUrArG rArArA rUrArG rCrArA rGrUrU rArArA rArUrA rArGrG rCrUrA rGrUrC rCrGrU rUrArU rCrArA rCrUrU rGrArA rArArA rGrUrG rGrCrA rCrCrG rArGrU rCrGrG rUrGrC mU*mU*mU* rU) and mouse Bdh1 (mA*mC*mG* rCrArG rGrCrA rUrCrU rCrArA rCrGrU rUrUrG rUrUrU rUrArG rArGrC rUrArG rArArA rUrArG rCrArA rGrUrU rArArA rArUrA rArGrG rCrUrA rGrUrC rCrGrU rUrArU rCrArA rCrUrU rGrArA rArArA rGrUrG rGrCrA rCrCrG rArGrU rCrGrG rUrGrC mU*mU*mU* rU) were purchased from IDT (m, 2O-methyl base