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Recurrent episodes in a separate pre-clinical OM model also demonstrated continuous microevolution of a haemoglobin-binding gene that resulted in a highly invasive NTHi phenotype that persisted for at least one month following clinical resolution of the infection (Harrison et al., 2020)

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Amersham enhanced chemiluminescence (ECL) detection kit (PRN2106) and nitrocellulose membranes (10600048) were purchased from GE Healthcare (Little Chalfont, UK)

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(Although multiple sclerosis (MS) is classically an autoimmune inflammatory demyelinating disease rather than a primary proteinopathy, we include it here due to overlapping chronic CNS injury and immune dysregulation influenced by the microbiome.) For each disease, specific microbial taxa, metabolites, and pathways implicated in pathogenesis are discussed

doi: 10.1371/journal.pone.0017599