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For siRNA-mediated gene silencing, M0-human macrophages or BMDMs were transfected with 25 nM of Silencer pre-designed siRNA targeting human LIPE /HSL (143993 and 143994, Life Technologies), Lipe mouse pre-designed siRNA Set A (HY-RS18621, MCE), or a non-targeting control pool (Dharmacon, Lafayette, CO) using Lipofectamine LTX Transfection Reagent (Thermo Fisher Scientific)

Subsequently, the membranes were washed three times with TBST and incubated with secondary antibody at room temperature for 2 h

doi: 10.17532/jhsci.2019.737 96 Rubio-AliagaI.WagnerC

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