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Results smarca5 -deficient RBCs tend to form aggregates in the CVP In our previously generated smarca5 zko1049a mutants (Ding et al., 2021), we observed that the blood clots were formed in CVP at 2 dpf, which was not present in their sibling embryos (Figure 1A)

Collagen & Leather 7 , 15 (2025)

Then, the small pieces were immersed in 5 mL collagenase IV (1 mg mL 1 ) with 0.2 mg mL 1 DNase I for 1 h at 37 C

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doi:10.1371/journal.pone.0001944.g001 For all data acquisition, high-order shim followed by automatic local shim adjustment was used and repeated until the half linewidth was accomplished under 3 Hz (phantom) or 8 Hz (human)

Jumping straight to high-dose NAD infusions can increase discomfort without providing additional benefit