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Methods Cell culture Human lung adenocarcinoma cell lines (A549, H322, H1299, H1792, H1975, H2009, PC9) were previously obtained 87 from the Harmon Cancer Center Collection (University of Texas-Southwestern Medical Center) or commercially from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium (Fisher Scientific, 11-875-119) supplemented with 5% FBS (Sigma-Aldrich, F0926) in the absence of antibiotics

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PRP (Platelet-Rich Plasma): When used in combination with other treatments for dark circles, such as carboxytherapy, PRP injections can significantly improve dark circles

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