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Protein expression was induced with 500 M IPTG for 3 h at 30 o C, and the heterodimer was purified to homogeneity using metal affinity chromatography and size-exclusion chromatography (SEC) using a Superdex 200 16/600 preparative column (Cytiva) 71,72

Fareleira, P., Santos, B.S., Antonio, C., Moradas-Ferreira, P., LeGall, J., Xavier, A.V., et al., Microbiology , 2003, vol

Additional investigation and guidance are needed for adult patients with idiopathic cholestasis [88]

Used to probe senescent-cell vulnerability and mechanisms by which senescent cells are maintained or cleared

Hudmon, A